At least 17 ducks and 11 chickens have died in the farm.
The virus was confirmed by the District Livestock Services Office (DLSO) after samples from the poultry farm tested positive. Khaltemasina was declared by the local administration as bird flu infected area.
The District Bird Flu Control Coordination Committee issued a 42-day ban on trade and movement of poultry to contain the virus.
A rapid response team led by Dr. Abadhesh Jha was tasked to cull the remaining infected poultry in the farm. Other birds within the 500-meter zone of Khaltemasina were also culled.
“Monitoring and sample testing have been carried out in other parts of Pokhara as well. Authorities are on high alert,” said Thagendra Prasad Aryal, information officer of DLSO.
Several cases of H5N1 have been found in Nepal since January 2013, particularly in poultry farms at Batulechaur, Simpani, Birauta and Sedibagar of Pokhara.